Fig. 5: Comparative enzymatic activity of AtD14, AtKAI2, RMS3, PsKAI2A and PsKAI2B proteins with GR24 isomers. | Communications Biology

Fig. 5: Comparative enzymatic activity of AtD14, AtKAI2, RMS3, PsKAI2A and PsKAI2B proteins with GR24 isomers.

From: Structural and functional analyses explain Pea KAI2 receptor diversity and reveal stereoselective catalysis during signal perception

Fig. 5

UPLC-UV (260 nm) analysis showing the formation of the ABC tricycle from GR24 isomers. The enzymes (10 μM) hydrolysis activity was monitored after incubation with 10 μM (+)-GR24 (yellow), (–)-GR24 (orange), (+)-2’-epi-GR24 (blue), or (–)- 2’-epi-GR24 (purple). The indicated percentage corresponds to the hydrolysis rate calculated from the remaining GR24 isomer, quantified in comparison with indanol as an internal standard. Data are means ± SE (n = 3). nd = no cleavage detected.

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