Fig. 1: Outline of the intersectional, anterograde transsynaptic targeting system. | Communications Biology

Fig. 1: Outline of the intersectional, anterograde transsynaptic targeting system.

From: Intersectional, anterograde transsynaptic targeting of neurons receiving monosynaptic inputs from two upstream regions

Fig. 1

a Schematic of the intersectional, anterograde transsynaptic targeting of neurons that receive monosynaptic inputs from two upstream brain regions. b EYFP fluorescence in 293T cells transfected with recombinase (pAAV-hSyn-Cre or pAAV-hSyn-Flpo-3×FLAG) and recombinase-dependent (pAAV-EF1α-DIO-EYFP or pAAV-EF1α-fDIO-EYFP) plasmid combinations. c EYFP fluorescence in 293T cells transfected with Cre, Flpo, and Con/Fon plasmid combinations. The pAAV-hSyn-Con/Fon-EYFP plasmid was transfected into all wells. AAV1 adeno-associated virus serotype 1, GOI gene of interest, EYFP enhanced yellow fluorescent protein.

Back to article page