Fig. 2: Anterograde transneuronal spread of AAV1-Cre and AAV1-Flpo. | Communications Biology

Fig. 2: Anterograde transneuronal spread of AAV1-Cre and AAV1-Flpo.

From: Intersectional, anterograde transsynaptic targeting of neurons receiving monosynaptic inputs from two upstream regions

Fig. 2

a Schematic of the anterograde transneuronal labelling. b–g Anterograde transneuronal spread of AAV1-Cre (b, d, f) and AAV1-Flpo (c, e, g) from the V1 to ipsilateral SC (b, c), retina to contralateral SC (d, e), and M2 to contralateral DS (f, g) pathways. The Cre (red) and 3×FLAG-tagged Flpo (red) were detected with anti-Cre and anti-FLAG antibodies, respectively. All images represent coronal sections, and the bottom images show the labelled EYFP (green)-positive somata in the SC (b–e) and DS (f, g). Blue, DAPI. N = 2 (b–e, g) and 3 (f) mice under each experimental condition. The numbers of EYFP-positive cells per section in either the SC (b–e) or DS (f, g) were as follows: 32.2 ± 41.8 (b), 43.5 ± 0.7 (c), 35.6 ± 7.0 (d), 27.3 ± 38.5 (e), 87.4 ± 10.0 (f), and 71.3 ± 10.0 (g). AAV1 adeno-associated virus serotype 1, EYFP enhanced yellow fluorescent protein, V1 primary visual cortex, SC superior colliculus, M2 secondary motor cortex, DS dorsal striatum.

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