Fig. 7: Memory T cell mediated antigen-specific T cell response in KRAS/LKB1 lung metastases in a humanized mouse model.

In-depth immune analysis was performed using multicolor flow-cytometry using a memory T cell panel in lung metastases and ex-vivo activation of memory T cells were determined by IFN-γ intracellular staining. a Strategy shown for in-vitro activation of T cells by tumor antigens after harvesting from tumor bearing treated humanized mice. b Scatter plots show the gating strategy of IFN-g positive T cells after challenging with tumor antigen. c Antigen-specific CD3+ T and CD8+ T cell responses determined by measuring the percentages of IFN-g expression upon stimulation with tumor antigens. d Scatter plots showing the level of IFN-γ expressing CD3+, CD4+, and CD8+ sorted CM/EM+ T cells in different treatment groups e gating strategy of analysis of sorted central memory and effector memory T cells. f Level of IFN-g expressing sorted EM/CM+ T cells and EM/CM⁻ T cells after stimulation with either tumor antigens by co-culturing with heat-killed A549 cancer cells or PMA under different treatment conditions. Statistics were shown at a significance level of p < 0.05 unless otherwise noted. Data are shown as mean percentage ± SD, n = 5. *P < 0.05, **P < 0.005, ***P < 0.0005.