Fig. 2: αβ T cells are the major producers of IL17A during the development of SR-induced hypersensitivity pneumonitis and ITK is not required for their ability to produce IL17A.

a WT non-IL17A-GFP reporter mice, or WT or Itk^−/− IL17A-GFP mice were exposed to SR as in Fig. 1, and frozen lung sections were analyzed for IL17A-GFP by fluorescence microscopy, Blue = DAPI staining, Green = IL17A-GFP⁺ cells indicated by white arrows. b WT IL17A-GFP mice were exposed to SR as in Fig. 1, and lung cells that are GFP⁺ were gated (i.e., GFP⁺ > cell type) and analyzed for numbers of αβ (filled circles), γδ (filled boxes) T cells and neutrophils (filled diamonds). (n = 3/group). c Proportion of GFP⁺ lung cells (i.e., GFP⁺ > cell type) from SR-exposed WT (filled symbols) or Itk^−/− (open symbols) IL17A-GFP mice that are αβ (circles), γδ (squares) T cells and neutrophils (diamonds) (n = 3/group). d WT (filled symbols) or Itk^−/− (open symbols) IL17A-GFP mice were exposed to SR as in Fig. 1, and αβ (circles) and γδ (squares) T cells were analyzed for the proportion that is IL17A-GFP⁺ (i.e., cell type > IL17A-GFP⁺) (n = 3–4/group). e Lung CD4⁺ (circles) and CD8⁺ αβ (triangles) T cells were analyzed for proportion IL17A-GFP⁺ (i.e., cell type > IL17A-GFP⁺) (n = 3–4/group).