Fig. 2: Fluorophore injection.
From: Injection into and extraction from single fungal cells
a Bright field and fluorescence images of the injection of lucifer yellow (LY) into S. cerevisiae, before, during, and after the injection. The images show a single oval microwell (in the center) containing two yeast cells; the right cell in the microwell was injected and was labeled, while the adjacent cell to its left was not injected and remained unlabeled. b S. pombe injected with LY. c Germ tube (left), hyphae (center left, center right), and pseudo-hyphae (right) of C.albicans injected with LY. d LY injections in C. cinerea hyphal compartments. The white arrows in (b), (c), and (d) indicate the injection sites. The asterisks in (c) and (d) indicate the vacuoles. The orange arrows in (d) indicate the septa of the injected hyphal compartment. Scale bars are 20 µm.
