Table 1 List of antibodies used in this study.
From: PDGF regulates guanylate cyclase expression and cGMP signaling in vascular smooth muscle
Antigene | Manufacturer | Cat. No. | Dilution |
|---|---|---|---|
Akt | Cell Signaling, Danvers, USA | 9272S | 1:1000 |
GAPDH | Cell Signaling, Danvers, USA | 2118S | 1:1000 |
Goat anti-Rabbit IgG AF-555 | ThermoFischer Scientific, Darmstadt | A-21428 | 1:500 |
IgG isotype control | Cell Signaling, Danvers, USA | 3900S | 0.1 µg (IP) |
Jagged-1 | Cell Signaling, Danvers, USA | 70109S | 1:1000 |
Anti-Mouse-HRP | Cell Signaling, Danvers, USA | 7076S | 1:10000 |
pAkt Serine 473 | Cell Signaling, Danvers, USA | 9271S | 1:1000 |
pRhoA Serine 188 | Santa Cruz, Santa Cruz, USA | Sc-32954 | 1:1000 |
Anti-Rabbit-HRP | Cell Signaling, Danvers, USA | 7074S | 1:5000 |
RhoA | Santa Cruz, Santa Cruz, USA | Sc-418 | 1:1000 |
sGCβ1 | Sigma-Aldrich, Munich, Germany | G4405 | 1:1000 (WB) 1:100 (Immunostaining) |
Tubulin | Dianova, Hamburg, Germany | DLN-09993 | 1:1000 |
Vimentin | Cell Signaling, Danvers, USA | 5741S | 1:1000 (WB) 1:100 (Immunostaining) 0.1 µg (IP) |
P-Rex1 | Cell Signaling, Danvers, USA | 13168S | 1:1000 (WB) |
