Fig. 2: Agonist-dependent and agonist-independent HA-β2-AR activity in WTT-CHO cells.
From: Atomic force microscopy-single-molecule force spectroscopy unveils GPCR cell surface architecture
a WTT-CHO cells were transfected with different amounts (0.1 or 1 µg) of HA-β2-AR-encoding vector (HA-β2-ARLow and HA-β2-ARHigh respectively) or the empty vector (control) and cAMP production was quantified following stimulation with 10 µM isoproterenol (ISO) for 30 min (a) or at basal state (b). When indicated, cells were pretreated with 200 mM sucrose for 1 h to prevent the receptor internalization process. Data represent the mean ± s.e.m. of four independent experiments, each performed in triplicates. The results are expressed as the difference in cAMP level measured in the presence and absence of isoproterenol (isoproterenol-promoted cAMP production) (a) or as basal cAMP levels (b). The statistical comparison was assessed using one-way ANOVA followed by Sidak’s multiple comparisons tests (*p < 0.05; **p < 0.01; ***p < 0.001; n.s. not statistically significant).
