Fig. 2: Tissue demasculinization upon CRE/lox excision of the transgenic Nix cassette. | Communications Biology

Fig. 2: Tissue demasculinization upon CRE/lox excision of the transgenic Nix cassette.

From: Transgenic expression of Nix converts genetic females into males and allows automated sex sorting in Aedes albopictus

Fig. 2

a Representative transgenic male pupa and male adult from the SM9 line. b Representative non-transgenic female pupa and female adult from the SM9 line. c Transgenic SM9 male pupa and adult injected as embryos in the posterior pole with CRE-recombinase that excised the Nix-eGFP cassette in the injected region. These individuals show a male anterior body with female genitalia. d Transgenic SM9 male pupa and adult injected with CRE-recombinase in the anterior pole of the embryo. Note the female anterior body and male genitalia. Scale bars in the bottom right corner of each picture represent 1 mm.

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