Fig. 3: CT are required for BDNF delivery to the hippocampus and increased Akt expression and signaling. | Communications Biology

Fig. 3: CT are required for BDNF delivery to the hippocampus and increased Akt expression and signaling.

From: Clathrin-nanoparticles deliver BDNF to hippocampus and enhance neurogenesis, synaptogenesis and cognition in HIV/neuroAIDS mouse model

Fig. 3: CT are required for BDNF delivery to the hippocampus and increased Akt expression and signaling.

Tat+ mice received i.p. Dox and one of four i.n. treatments (BDNF, Sal, CT, or BDNF-CT) daily for 4 days (a). Global control (Tat−) mice received i.p. and i.n. saline (Tat-/Sal). The hippocampal tissue shows increased mBDNF (b), proBDNF (c), mBDNF/proBDNF ratio (d), Akt (e), pAkt (f), and the pAkt/Akt ratio (g) in Tat+ mice that received BDNF-CT vs. other treatments (*P < 0.05, **P < 0.01 and ***P < 0.001), as well as vs. saline treated Tat- mice (#P < 0.05 and ##P < 0.01, ###P < 0.001). Tat+ mice treated with BDNF-CT or Sal had higher pAkt/Akt ratios (g) compared to Tat- controls but levels of Akt and pAkt were significantly higher in BDNF-CT vs. Sal treated Tat+ mice indicating enhanced Akt expression and signaling only with BDNF-CT NPs. Representative WB images are shown (h, i). Data are shown as the mean % change from Tat- control (Tat-/Sal) represented by dotted lines (n = 4–5 per group, five cohorts were processed in parallel). Error bars represent S.E.M.

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