Fig. 5: BDNF-CT rescues neurogenesis from Tat-induced toxicity in the hippocampus. | Communications Biology

Fig. 5: BDNF-CT rescues neurogenesis from Tat-induced toxicity in the hippocampus.

From: Clathrin-nanoparticles deliver BDNF to hippocampus and enhance neurogenesis, synaptogenesis and cognition in HIV/neuroAIDS mouse model

Fig. 5: BDNF-CT rescues neurogenesis from Tat-induced toxicity in the hippocampus.

Tat+ mice received i.p. Dox and i.n. saline or BDNF-CT nanoparticles (0.3 mg/kg of BDNF and 2.4 mg/kg of CT) daily for 7 days. Tat− controls received i.p. and i.n. saline. All mice received BrdU (50 mg/kg, i.p. every 12 h) on days 1 and 2 and were sacrificed on the 7th day (a). Hippocampal sections containing the granule cell layer were evaluated and double labeled cells (BrdU+ and DCX+) are expressed as the percentage of BrdU+ cells (b). The mean percentage was higher in BDNF-CT-treated Tat+ mice vs. matched controls (Tat+ and Tat−) that received saline, and was lower in Tat+ vs. Tat− mice. BrdU+ cells (red, c–e), DCX+ cells (green, f–h) and double labeled cells (BrdU+ and DCX+) (i–k) are shown. Nuclei (blue) were labeled with DAPI. Data are represented as the mean percentage. Error bars represent S.E.M. (n = 3 per group; *P < 0.05, **P < 0.01, and ***P < 0.001). Scale bars are 50 µm.

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