Fig. 3: The effect of EBI2 signaling on brown adipocytes respirometry and ROS generation. | Communications Biology

Fig. 3: The effect of EBI2 signaling on brown adipocytes respirometry and ROS generation.

From: EBI2 is a negative modulator of brown adipose tissue energy expenditure in mice and human brown adipocytes

Fig. 3

a In vitro respirometry of brown adipocytes pre-treated with 7α,25-OHC (1 µM), and EBI2−/− brown adipocytes (n = 6 independent samples). b–h NE-induced O2 consumption (b), maximal respiration (c), spare respiratory capacity (d), basal respiration (e), ATP production (f), proton leak (g) and non-mitochondrial respiration (h) of brown adipocytes pre-treated with 7α,25-OHC (1 µM), and EBI2−/− brown adipocytes (n = 6 independent experiments). i ROS production in brown adipocytes after 1 h treatment with NE (1 µM), 7α,25-OHC (1 µM), NIBR189 (10 µM) or 3% H2O2 (n = 4 independent samples). Mean ± s.e.m., one-way ANOVA with Tukey post-hoc test and Student’s t test, *p < 0.05, **p < 0.01, ****p < 0.0001.

Back to article page