Fig. 2: Crystal structure of MW01 and MW05 Fab in complex with SARS-CoV-2 RBD.

a The overall structure of MW01 and MW05 Fab in complex with SARS-CoV-2 RBD. The SARS-CoV-2 RBD is colored in gray. Heavy chain and light chain of MW01 are shown as hot pink and orange cartoons. Heavy chain and light chain of MW05 are shown as dark green and light green cartoons. b Superimposition of MW01/RBD or MW05/RBD with hACE2/RBD. The SARS-CoV-2 RBD is colored in gray surface. Heavy chain and light chain of MW01 and MW05 are presented in cartoon with the same colors in Fig. 2a. hACE2 is colored in salmon. c Superimposition of MW01, MW05, and MW07 Fab to partially open SARS-CoV-2 S trimer (PDB code: 6VYB) with 1 “up” RBD and 2 “down” RBDs. For MW01 and MW05, one Fab is superposed to the “up” RBD, and one Fab is superposed to one of the “down” RBDs. Superimposition of Fab to the other “down” RBD will introduce clashes. MW07 Fab can only be superposed to the “open” RBD with no clashes. The heavy chain and light chain of MW01 and MW05 are shown with same colors as Fig. 2a. The heavy chain and light chain of MW07 are colored in blue and light cyan, respectively. The three chains of SARS-CoV-2 S trimer are shown as wheat, gray and yellow surface. d Superimposition of MW01, MW05, and MW07 to SARS-CoV-2 S trimer with 3 “up” RBDs (PDB code 7A98). For all these three mAbs, three Fabs are superposed to three “up” RBDs in SARS-CoV-2 trimer. MW01, MW05, MW07 and S trimer as shown with the same colors as Fig. 2c.