Fig. 5: Effect of 12 h of TGFβ treatment on GSI changes (∆GSI).
From: Co-transcriptional splicing efficiency is a gene-specific feature that can be regulated by TGFβ
a Scatter plot of nascent pre-mRNA level changes (log2FC) versus GSI changes (∆GSI) after 12 h of TGFβ. Genes with decreased GSI (∆GSI < –0.5, p < 0.05) or increased GSI (∆GSI > 0.5, p < 0.05) are depicted in red or blue, respectively. b Boxplot of nascent pre-mRNA level changes (log2FC) after 12 h of treatment with TGFβ versus vehicle in genes with decreased GSI or increased GSI. Unpaired Student’s t-test p-values are shown. c, d GSI levels at the three conditions tested (vehicle or 2 and 12 h of TGFβ-treated at the 2 h or 12 h time point) for genes with a decreased GSI (c) or an increased GSI (d) after 12 h of TGFβ. Paired Student’s t-test p-values of the comparison between the indicated distributions are shown. Sample size (n) of all set of data are provided in Supplementary Data 4.
