Fig. 3: RNA sequence KUCaP2 AD and CR tumors. | Communications Biology

Fig. 3: RNA sequence KUCaP2 AD and CR tumors.

From: Comprehensive genomics in androgen receptor-dependent castration-resistant prostate cancer identifies an adaptation pathway mediated by opioid receptor kappa 1

Fig. 3

Among 30 most significantly upregulated genes in RNA seq of KUCaP2 CR compared with AD tumors, seven genes that were reported to be frequently amplified in PCa were picked up. Likewise, among 30 most significantly downregulated genes in KUCaP2 CR tumors, five genes that were reported to be frequently altered (deep deletion, and truncating and missense mutations) in CRPC were picked up. a Dot plot of log10[fkpm] indicating the 12 genes. b Landscape of alterations (amplification, deep deletion, and truncating and missense mutations) of the 12 genes primary PCa24. c Summarized results from integrated genomics including RNA seq of KUCaP2 tumors (AD vs CR), cDNA microarray of KUCaP2 tumors (AD vs CR)16, ChIP seq of KUCaP2 tumors (AD and CR), and ChIP seq of AILNCaPs (four independent sublines) and LNCaP cells cultured in FBS (LNCaP_FBS), charcoal-stripped FBS (LNCaP_CSFBS), and CSFBS supplemented with 1 nM dihydrotestosterone (LNCaP_CSFBS + DHT). d–j Quantitative RT-PCR in human PCa cell lines. Expressions of TRPA1 (d), CP (e), CLSTN2 (f), MGLL (g), OPRK1 (h), NMNAT2 (i), and ROBO1 (j) normalized by that of GAPDH in the indicated PCa cell lines.

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