Fig. 5: SENTINEL on 50 blinded nasopharyngeal swabs from patients.
From: Improving the specificity of nucleic acid detection with endonuclease-actuated degradation
a Schematic of SARS-CoV-2 viral particle detection using SENTINEL from patient nasopharyngeal swabs in viral transport media (VTM). b Graph of SENTINEL score versus Cq from qPCR for the 50 blinded patient samples (25 with detectable virus from qPCR—Cq below 40, 25 negative controls—Cq above 40). qPCR Cqs were initially measured from 300 µL of patient sample, and adjusted here to be consistent with the 2.5 µL of patient samples used for SENTINEL by adding log2(300/2.5) = 6.9. The four red labels divide the graph into True Positives (‘TP’), False Positives (‘FP’), True Negatives (‘TN’), and False Negatives (‘FN’). c True positives with SENTINEL (‘+’) had significantly lower qPCR Cq compared to false negatives (‘–’) (p < 0.001) from Student’s t-test. d Receiver operating characteristic (ROC) curves using SENTINEL versus RT-LAMP alone.
