Fig. 7: RT-qPCR validation of hCRISPR expression in normal prostate tissue and tumor samples from four prostate cancer patients and detection by SHERLOCK and DETECTR. | Communications Biology

Fig. 7: RT-qPCR validation of hCRISPR expression in normal prostate tissue and tumor samples from four prostate cancer patients and detection by SHERLOCK and DETECTR.

From: CRISPRs in the human genome are differentially expressed between malignant and normal adjacent to tumor tissue

Fig. 7: RT-qPCR validation of hCRISPR expression in normal prostate tissue and tumor samples from four prostate cancer patients and detection by SHERLOCK and DETECTR.

a Expression of hCRISPR chr19_106 and chr9_209. Left panels show the normalized number of sequencing reads mapping to hCRISPR chr19_106 and chr9_209 in the NGS-ProToCoL RNA-Seq dataset. Right panels show the normalized transcript copy number of hCRISPR chr19_106 and chr9_209 measured by RT-qPCR in the same samples. NAP is normal adjacent to tumor prostate tissue. Tumor, prostate tumor tissue. b Photograph of the DETECTR technology identifying the presence of hCRISPR chr19_106. (+) indicates a positive and (−) indicates a negative detection result. c Similar to b but for the presence of hCRISPR chr9_209.

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