Fig. 4: Thermal denaturation analysis of SR with ATP and malonate.

a Thermal denaturation curves of SR with and without 1 mM ATP and malonate. A right-shift indicates an increase in Tm and thermostability. The small right-shift and thermostability increase with the addition of malonate alone is augmented significantly when both ATP and malonate are present. b Melting temperatures of SR across a concentration series of malonate (0–1 mM) with and without 1 mM ATP. Tm shifts with malonate alone are negligible across the series; 1 mM ATP appears to have a destabilising effect at low concentrations of malonate before sufficient levels are reached to drive complex formation, resulting in a high Tm shift. The experiment was conducted in triplicate and repeated five individual times. Data points represent the mean of means, and error bars show standard error of the mean (Error bars = SEM, n = 5).