Table 2 cloning primer for lentivirus plasmids.

From: A VSV-based assay quantifies coronavirus Mpro/3CLpro/Nsp5 main protease activity and chemical inhibition

Name

Sequence (5'-3' direction)

LV-L-for

CTGTTTTGACCTCCATAGAAGATTCTAGAGCTAGCATGGAAGTCCACGATTTTGAG

L-LV-rev

GAGGGAGAGGGGCGGATCCCCTTAATTAATTAATCTCTCCAAGAGTTTTCCTC

LV-GFP-for

GACCTCCATAGAAGATTCTAGAGCTAGCATGAGCAAGGGCGAGGAACTG

L-33bp-after-HpaI-rev

GATGTTGGGATGGGATTGGC

LV-P-for

CTGTTTTGACCTCCATAGAAGATTCTAGAGCTAGCATGGATAATCTCACAAAAGTTC

P-LV-rev

GAGGGAGAGGGGCGGATCCCCTTAATTAACTACAGAGAATATTTGACTCTCGC

GGSG-BatCoV-On-for

GGCGGAAGCGGCGGAGGGAGCGGGGGCGGGAGCGGAAGCGTCGCCAGTGCTGCGC

BatCoV-GGSG-On-rev

GCCGGATCCACCGCCTGAGCCGCCTCCGGACCCTCCTCGAAACATAGATTGAAATTTACCTTG

GGSG-HCoV-229E-On-for

GGCGGAAGCGGCGGAGGGAGCGGGGGCGGGAGCGGAGTATCTTATGGCTCAACGCTCCAA

HCoV-229E-GGSG-On-rev

GCCGGATCCACCGCCTGAGCCGCCTCCGGACCCTCCAAACATGGATGTAGTCTTACCAGATTG

GGSG-HCoV-NL63-On-for

GGCGGAAGCGGCGGAGGGAGCGGGGGCGGGAGCGGAATCAGTTACAATAGTACCTTGCAAAGC

HCoV-NL63-GGSG-On-rev

GCCGGATCCACCGCCTGAGCCGCCTCCGGACCCTCCAAGCCCGAATATAACCTTTCCTG

GGSG-MERS-On-for

GGCGGAAGCGGCGGAGGGAGCGGGGGCGGGAGCGGATCAATCACTAGCGGTGTATTGC

MERS-GGSG-On-rev

GCCGGATCCACCGCCTGAGCCGCCTCCGGACCCTCCGTATGTTACTTTTCTTACACCGGAC

GGSG-MHV-A59-On-for

GGCGGAAGCGGCGGAGGGAGCGGGGGCGGGAGCGGATCAGTCACCACTTCATTTCTCC

MHV-A59-GGSG-On-rev

GCCGGATCCACCGCCTGAGCCGCCTCCGGACCCTCCTTTTATTACTCTAGTCCTTTTACTCTGCAG

GGSG-Polio-On-for

GGCGGAAGCGGCGGAGGGAGCGGGGGCGGGAGCGGAACCATTCGGACAGCAAAGGTAC

Polio-GGSG-On-rev

GCCGGATCCACCGCCTGAGCCGCCTCCGGACCCTCCAGGTCTCATCCACTGGATTTC

GGSG-SARS1-On-for

GGCGGAAGCGGCGGAGGGAGCGGGGGCGGGAGCGGAAGTATCACGTCTGCTGTGC

SARS1-GGSG-On-rev

GCCGGATCCACCGCCTGAGCCGCCTCCGGACCCTCCTTTGACTATTTTTTTGAACTTACCTTG

BatCoV-On-N-term-QtoN-for

GGCGGAAGCGGCGGAGGGAGCGGGGGCGGGAGCGGAAGCGTCGCCAGTGCTGCGCTCAACGCGGGTCTTACTC

HCoV-229E-On-N-term-QtoN-for

GGCGGAAGCGGCGGAGGGAGCGGGGGCGGGAGCGGAGTATCTTATGGCTCAACGCTCAACGCCGGCTTGCGC

HCoV-NL63-On-N-term-QtoN-for

GGCGGAAGCGGCGGAGGGAGCGGGGGCGGGAGCGGAATCAGTTACAATAGTACCTTGAACAGCGGACTG

MERS-On-N-term-QtoN-for

GGCGGAAGCGGCGGAGGGAGCGGGGGCGGGAGCGGATCAATCACTAGCGGTGTATTGAACAGTGGTTTGGTC

MHV-On-N-term-QtoN-for

GGCGGAAGCGGCGGAGGGAGCGGGGGCGGGAGCGGATCAGTCACCACTTCATTTCTCAACTCCGGGATAG

Polio-On-N-term-QtoN-for

GGCGGAAGCGGCGGAGGGAGCGGGGGCGGGAGCGGAACCATTCGGACAGCAAAGGTAAACGGACCAGGGTTC

SARS1-On-N-term-QtoN-for

GGCGGAAGCGGCGGAGGGAGCGGGGGCGGGAGCGGAAGTATCACGTCTGCTGTGCTCAACTCAGGCTTCAG

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