Fig. 3: Viscoelastic mapping of the foregut region reveals different biomechanical properties of neighboring progenitor populations.

a 2D projection of viscoelasticity map of the liver, gut, and yolk. The color scale on the left indicates cellular viscoelasticity: increasing blue values show more elastic tissues, while increasing red values represent more viscous tissues (n = 178 nanoparticles, N = 32 embryos). Scale bar: 20 µm. b Viscoelasticity map of the foregut region in a projection orthogonal to (a) and using color code corresponding to average α values for each tissue type, color scale bar as in (a). c Quantification of α values for different tissues shown in a box plot; n = number of analyzed particles, N = number of embryos. d Statistical comparison of α value distributions between different tissues. The table provides the P value calculated for each pair of tissues using a two-tailed equal variance Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001.