Fig. 4: Glycan array of VP8*B and molecular docking of the selected glycan. | Communications Biology

Fig. 4: Glycan array of VP8*B and molecular docking of the selected glycan.

From: Novel fold of rotavirus glycan-binding domain predicted by AlphaFold2 and determined by X-ray crystallography

Fig. 4

a The glycan array result of GST-tagged VP8*. The X-axis represents the CFG glycan number, and the Y-axis represents the relative fluorescence units (RFUs) of binding. Standard deviation from the mean of 6 replicates of each glycan printed on the array is provided (n = 6). b The glycan structures with the highest binding signals were drawn using GlycoGlyph49. c Molecular docking of the LacNAc disaccharide (Gal-GlcNAc) onto VP8*B. The highest-ranked docked glycan is shown in the stick model, and the protein is shown with a transparent surface. The inset shows how the glycan interacts with VP8*B residues. The glycan and the VP8*B residues are shown in stick and ball-and-stick models, respectively.

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