Fig. 1: M1 enhances the lability of mNT and NAF-1’s [2Fe–2S] clusters. | Communications Biology

Fig. 1: M1 enhances the lability of mNT and NAF-1’s [2Fe–2S] clusters.

From: An anti-diabetic drug targets NEET (CISD) proteins through destabilization of their [2Fe-2S] clusters

Fig. 1

a Structure of the M1 molecule. The [2Fe-2S] cluster-release from 20 µM of mNT (b) or 20 µM NAF-1 (d) NEET proteins was monitored by UV-Vis absorption spectroscopy. The 458 nm characteristic absorption peak of [2Fe–2S] clusters of the NEET proteins which were incubated with- (empty circle) or without (filled circle) 60 µM of M1 molecule, at a temperature of 37 °C and pH 6.0. Error bar represents the standard deviation of three independent experiments. ITC binding curve of M1 to mNT (c) or to NAF-1, (e). 50 µM of proteins (mNT or NAF-1) were titrated against 500 µM of M1 by injecting 3 µL for 13 injections. Each measurement was repeated in three independent experiments.

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