Fig. 3: NK cells (blue) penetrated into the GSLC tumorospheres (green) and directly interacted with tumor cells. | Communications Biology

Fig. 3: NK cells (blue) penetrated into the GSLC tumorospheres (green) and directly interacted with tumor cells.

From: Infiltrating natural killer cells bind, lyse and increase chemotherapy efficacy in glioblastoma stem-like tumorospheres

Fig. 3

IL-2 activated primary NK cells (a, b) or super-charged NK cells (c) were added to NCH421k tumorospheres in ratio 2.5:1 for 24 h before images were taken by confocal microscope. Single focal plane (z = 33 μm) showed penetration of NK cells into the GSLC spheroid and co-localization of blue and green cells within spheroids (upper b, c). Spot analysis in Imaris software of selected areas, where GSLC and NK cell signals co-localized (arrows), revealed direct NK-GSLC interactions (lower b, c). Surface rendering of GSLCs and NK cells in Imaris showed direct cellular interactions (d). Gray surfaces represents areas of green GSLC surfaces touching blue surface of NK cells (arrow). Red dots represent PI-positive dead cells. Scale bar = 100 μm (upper b, c), 10 μm (lower b, c) and 20 μm (d).

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