Fig. 7: Super-charged NK cell-mediated lysis was impaired after GSLC differentiation with 10% FBS and exposure to IFN-γ. Differentiation and IFN-γ treatment decreased GSLC proliferation and altered levels of ligands for NK cell activating and inhibitory receptors as well as decreased levels of OLIG2. | Communications Biology

Fig. 7: Super-charged NK cell-mediated lysis was impaired after GSLC differentiation with 10% FBS and exposure to IFN-γ. Differentiation and IFN-γ treatment decreased GSLC proliferation and altered levels of ligands for NK cell activating and inhibitory receptors as well as decreased levels of OLIG2.

From: Infiltrating natural killer cells bind, lyse and increase chemotherapy efficacy in glioblastoma stem-like tumorospheres

Fig. 7

After NCH421k cells were treated with cell culture medium containing 10% FBS (dark gray bars) for 7 days or 30 ng of recombinant human IFN-γ (light gray bars) for 48 h, cytotoxicity assay using calcein AM release (a), doubling time analysis (b) and protein expression analysis using immunostaining and flow cytometry (c) were performed. Neurobasal GSLC culture media was used as control (black bars). Data are presented as means ± SEM, data points represent independent biological replicates, n ≥ 3.

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