Fig. 1: Short-term growth in BSC-GM mixed with B8. | Communications Biology

Fig. 1: Short-term growth in BSC-GM mixed with B8.

From: Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat

Fig. 1

a BSC proliferation over 3 & 4 days in mixtures of BSC-GM (20% FBS) and B8 media. At the four-day timepoint, mixtures of up to 62.5% B8 significantly improved growth compared to BSC-GM alone, and mixtures of up to 87.5% B8 did not significantly reduce growth (p = 0.27). B8 alone showed a significant reduction in growth over four days compared with BSC-GM alone, and showed stagnating growth after three days. n = 6 distinct samples; statistical significance was calculated by one-way ANOVA on day 4 data comparing all samples with BSC-GM controls, and is indicated by asterisks, in which p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), and p < 0.0001 (****). b Brightfield images of BSCs grown for three days in BSC-GM or B8 media. Images show that cell morphology was consistent in serum-containing or serum-free conditions. Cell confluency in the images is qualitatively consistent with growth analysis in part (a). Scale bars are 200 μm.

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