Fig. 4: Short-term growth in B8 & Beefy-9 with reduced FGF-2. | Communications Biology

Fig. 4: Short-term growth in B8 & Beefy-9 with reduced FGF-2.

From: Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat

Fig. 4

a BSC proliferation over 4 days in B8 or Beefy-9 with various concentrations of FGF-2. FGF-2 could be reduced to 5 ng/mL or 1.25 ng/mL in B8 or Beefy-9, respectively, without significantly impacting cell growth over four days. n = 6 distinct samples; statistical significance was calculated by one-way ANOVA with multiple comparisons between various FGF-2 concentrations and 40 ng/mL control conditions. Lack of significance between samples is indicated by ‘ns’ across all samples that hold no significant difference from 40 ng/mL). b Brightfield images of BSCs grown for three days in Beefy-9 media with 0 or 5 ng/mL FGF-2. Images show that the complete removal of FGF-2 from Beefy-9 significantly affects satellite cell morphology, whereas a reduction to 5 ng/mL did not affect morphology as compared with images in Figs. 1 and 2. Scale bars are 200 μm.

Back to article page