Fig. 6: BSC Myogenicity over long term culture. | Communications Biology

Fig. 6: BSC Myogenicity over long term culture.

From: Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat

Fig. 6

a Immunofluorescent staining for Myosin Heavy Chain (MF20, green) in BSCs at passages 2, 4 and 6 in various media. Cells were cultured to confluency and differentiated in a serum-free differentiation medium as previously described. Images revealed that myogenicity of BSCs was maintained throughout long term culture in both serum containing and serum-free media. Scale bars are 200 μm. b Quantitative PCR for Pax3, MyoD, Myogenin (MyoG) and Myosin Heavy Chain (MHC) expression in differentiated cells shows no significant difference between media types. Results are supported by immunofluorescent staining for these markers (Fig. S6). n = 3 distinct samples; statistical significance was calculated via two-way ANOVA with multiple comparisons between media.

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