Fig. 6: In real neuronal data, deconvolution-based estimates of spike transmission gain are especially higher in the presence of burst spiking activity.

a–c Data were recorded from hippocampal region CA1 of three freely-moving mice using high-density silicon probes. a Example wideband data (band-pass filtered 0.1–7500 Hz) recorded by two sites on the same shank, separated vertically by 60 μm. Orange and brown traces and tick marks correspond to PYR and INT spikes, respectively. b Cumulative distributions of the firing rates of n = 1041 PYR and n = 215 INT (solid lines). Dashed lines show distributions of n = 611 PYR and n = 152 INT used for further analyses (d, e). c Distributions of burst indices of the same four neuronal groups. d In PYR-to-INT pairs with putative monosynaptic connections, STG estimates based on deconvolution are higher than estimates that employ only median filtering. Scatter plot shows eSTG_dc-median vs. eSTG_median for all n = 7991 PYR-to-INT pairs in which both eSTGs were positive. PYR-to-INT pairs with a consistent monosynaptic CCH peak, in which the PYR also exhibits a non-negative burst index, are denoted as “analysis pairs” (pink dots; n = 1274 pairs). For the analysis pairs, the eSTG_dc-median is higher than the eSTG_median (p < 0.001, Wilcoxon’s paired signed-rank test). e Four example CCHs with the median predictor. Insets: The ACHs of the putative presynaptic neurons, which exhibit burst spiking. f dcCCHs for the pairs in (e). Insets: Conditional rate (CR) histograms based on the CCHs (purple) and the dcCCH (green). In all examples, the deconvolution-based eSTG is higher than the eSTG estimated without deconvolution. g eSTG ratios for the full dataset (n = 1274 pairs), shown as a function of the presynaptic burst index. Deconvolution-based estimates are consistently higher than estimates without deconvolution (p < 0.001, Wilcoxon’s signed-rank test). Estimates are particularly higher when spike bursting is more prevalent (p < 0.001, permutation test).