Fig. 3: Effects of L-proline on the regulatory activity of PutA.
From: Proline utilization A controls bacterial pathogenicity by sensing its substrate and cofactors
a The effect of L-proline on epsA gene expression was analysed by assessing the β-galactosidase activity of the epsA-lacZ transcriptional fusions in the wild-type and putA mutant strains with the addition of different concentrations of L-proline. b Effect of L-proline on EPS production in the wild-type strain. c EMSA analysis of the effect of L-proline on the binding of PutA to the epsA promoter in vitro. A biotin-labelled 336-bp epsA promoter DNA probe was used for the protein binding assay. The protein was incubated with the probe in the presence of L-proline at different concentrations at room temperature for 30 min. Results in a, b are mean ± standard deviations of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001 (unpaired t-test). In c experiment was performed three times and representative images from one experiment are shown.
