Fig. 6: Influence of PutA on the pathogenicity of R. solanacearum in tomato plants.
From: Proline utilization A controls bacterial pathogenicity by sensing its substrate and cofactors
CFUs of the R. solanacearum wild-type, wild-type treated with 1 mM L-proline and 4 mM L-glutamic acid, respectively, putA mutant and complemented strains in the roots (a) and stems (b) of tomato plants. The effects of putA on the virulence of R. solanacearum were measured by assessing the disease index of bacterial wilt in tomato (c). Unwounded tomato plants were subjected to soil-soak inoculation with 8 mL of bacterial culture (OD600 = 1.0) and incubated at 28 ± 1 °C under a 12-h light/dark cycle with a relative humidity (RH) of 90%. Results in a, b and c are mean ± standard deviations of three biologically independent samples. *p < 0.05; **p < 0.01; ***p < 0.001 (unpaired t-test).
