Fig. 1: HCT116 and RKO GSK5032-resistant cells are globally demethylated. | Communications Biology

Fig. 1: HCT116 and RKO GSK5032-resistant cells are globally demethylated.

From: Chromosome-specific retention of cancer-associated DNA hypermethylation following pharmacological inhibition of DNMT1

Fig. 1

a Dose dependency curves showing that HCT116 and RKO cells long term treated with high doses of GSK5032 become resistant. Each point represents a percentage of surviving cells compared to cells treated with a vehicle control (n = 5 for HCT116 parental, n = 7 for HCT116 GSK5032-resistant, n = 3 for RKO parental, and n = 3 for RKO GSK5032-resistant). b DNA methylation density curves showing the distribution of the β-values on the Infinium EPIC methylation array (n = 627,029 probes). Probes with detection pval > 0.05 in any sample were removed from the analysis. β-values range from 0.0 to 1.0 where a β-value of 0.0 indicates completely unmethylated and a β-value of 1.0 indicates complete methylation. c Box plot of DNA methylation (β-value) distribution of all EPIC array probes for each individual sample queried in (b). Center line, median; box limits, upper and lower quartiles; whiskers, 5 and 95% percentile; ‘+’, mean; outliers removed for visualization purposes (n = 627,029 probes).

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