Fig. 4: Hypermethylation of CpGs that are unmethylated in non-cancer human tissues is replicated in TCGA samples for colon and rectal adenocarcinoma as well as other cancer types.

Heatmaps showing DNA methylation level as measured by the 22 HCT116 GSK5032-resistant probes (rows) in (a) COAD/READ samples (columns) (n = 402 COAD/READ tumor samples, n = 41 adjacent normal samples) (b) and a panel of cancer samples other than COAD/READ from TCGA data for identified probes that were unmethylated in non-cancer human tissues. A color spectrum of blue to red indicates low to high levels of DNA methylation (β-values ranging from 0 to 1). Samples were organized first by biological sex and then tissue type (cancer/normal). Genes discussed in the main text are labeled in red. Tumor type annotation is plotted beneath each heatmap, with color code explained as ‘source’ (n = 8790 human tumor samples, n = 714 adjacent normal samples).