Fig. 4: The knockdown of uS7 reduces the CDK6 protein in colon cancer cell lines.

a–d HT-29 cells were transfected with negative control siRNA (siCtrl) or two different siRNAs targeting uS7 (siuS7 #1 and #2). After 48 h, the depletion of uS7 was confirmed (a). After 72 h, cell proliferation was examined using the CCK-8 assay (b). After 48 h, the cell cycle was analyzed by flow cytometry (c), and protein levels were investigated by western blotting (d). e SW480 cells were transfected with each siRNA. After 48 h, cells were lysed and subjected to western blotting. In d, e, the signal of each western blot was quantified using ImageJ software and normalized by the value of β-actin. The value of each signal was indicated below the blot. Data are means ± S.D. (n = 3 biologically independent experiments). *P < 0.05 significantly different from siCtrl.