Fig. 4: Four-way ANOVA analysis of differences in resistance to oxidative stress depending on sex (SEX), age (AGE), genotype (GT), and combination of interventions (COND), including dietary restriction (DR), geroprotectors (3G), low ambient temperature (18 °C), and maintenance in the dark (DD).

a w/w males, b E(z)/w males, c w/w females, d E(z)/w females. ^###p < 0.001—differences between males and females; ^§p < 0.05, ^§§§p < 0.001—differences between w/w and E(z)/w genotypes; *p < 0.05; **p < 0.01; ***p < 0.001—differences between control and experimental maintaining conditions; ^&&&p < 0.001—differences between ages. The level of statistical significance of differences was revealed by four-way ANOVA with poct-hoc Bonferroni tests. The error bars show standard error; n = 96 flies. All source data underlying the graphs are presented in the Supplementary Data 1.