Fig. 10: DENV RNA is modified by m6A in infected mosquito cells.
a MeRIP-RT-qPCR analysis of RNA harvested from DENV-infected Aag2 cells (72 hpi) and immunoprecipitated with anti-m6A or IgG using primers to DENV genome. For the extracellular virions, the supernatant collected from DENV-infected cells was treated at room temperature with 17 μg of RNase A per ml for 20 min to remove any naked RNA. Eluted RNA was quantified as a percentage of input. m6A Control, positive control RNA with m6A modification; unmodified, negative control RNA without m6A modification. Error bars represent mean ± S.D. *p ≤ 0.01, ***p ≤ 0.001 (t-test). b Map of m6A-binding sites in the DENV RNA genome by MeRIP-Seq of RNA isolated from DENV-infected Aag2 cells based on three biological replicates. Read coverage, normalized to the total number of reads mapping to the viral genome for each experiment, is in red for MeRIP-Seq and in blue for input RNA-Seq. The five identified peaks (P1-P5) are indicated on the graph.
