Table 1 Criteria for events to be classified as true single EVs by IFCM.

From: An imaging flow cytometry-based methodology for the analysis of single extracellular vesicles in unprocessed human plasma

#

Criteria

Reasoning

1

Standardization of SSC signals

Allows estimation of particle sizes

2

Single Spot Fluorescence

Single-particle analysis/no coincidence events

3

Colocalization of fluorophores

Indicating the presence of markers in the same particle/event

4

Signal disappears after Detergent Treatment

Confirmation that detected events are of biological origin

5

Linear correlation with Dilution factor

Single-particle analysis & confirmation that events are biological

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