Fig. 1: Edwardsiella tarda TraT is essential to serum resistance and complement activation.
From: Edwardsiella tarda TraT is an anti-complement factor and a cellular infection promoter
a E. tarda wild type (TX01), traT mutant (TX01ΔtraT), and traT complement (TX01ΔtraT/traT) strains were treated with serum for 1 h, and bacterial survival was then determined. Escherichia coli was included for comparison. Different letters indicate statistical significance among the samples, P < 0.05. b E. tarda variants and E. coli were treated with or without (control) serum as above and subjected to transmission electron microscopy. Bar, 1 μm. c–f E. tarda variants and E. coli were incubated with diluted serum for 1 h. The hemolytic activity (c), bactericidal activity (d), C5a concentration (e), and chemotactic activity (f) in the serum were determined. In all panels except (b), data are the means of three experiments and shown as means ± SEM. For panels c, d, e, and f, in each serum dilution, different letters indicate statistical significance among the samples, P < 0.05.
