Fig. 1: Characterization of QD-α-syn seeds. | Communications Biology

Fig. 1: Characterization of QD-α-syn seeds.

From: Quantum-dot-labeled synuclein seed assay identifies drugs modulating the experimental prion-like transmission

Fig. 1

a Regions of interest (ROI) (white rectangles) for immunohistochemical stainings with antibodies at 1 and 6 h after QD-α-syn-seed or QD-sa-α-syn-monomer injection. iSt ipsilateral striatum (injection side), iCo ipsilateral cortex, CC corpus callosum, cCo contralateral cortex (opposite side of injection), cSt contralateral striatum. Bar: 500 μm (a). b QD-α-syn-seeds (purple) and anti-EEA1 immunoreactivity (green). White: colocalization. Blue: DAPI. c–g Other markers (green): LAMP1 (c), LAMP2 (d), VAMP2 (e), 22C11 for APP (f), LRRK2 (g). Quantitative analyses are shown in Supplemental Fig. S3. h No QD-sa-α-syn monomer in entorhinal cortex(EC). j QD-α-syn seeds in EC. i No QD-sa-α-syn monomer in Amygdala (Amy). k QD-α-syn seeds in Amy. l No QD-sa-α-syn monomer in DARPP32 positive medium spiny neurons in cSt. n Localization of QD-α-syn seeds in DARPP32 positive medium spiny neurons in cSt. m No QD-sa-α-syn monomer in tyrosine hydroxylase positive neuron in SNc. o Localization of QD-α-syn seeds in tyrosine hydroxylase positive neuron in SNc. Yellow arrows: QD-α-syn seeds in cSt (n) and SNc neurons (o). Bar: 5 μm (b–o).

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