Fig. 2: QD-α-syn seed dynamics and localization in the brain slices.
a Experimental procedures for time lapse imaging of slice. Slices were half submerged in culture medium and tightly attached at the bottom of six-well plate glass bottom dish. Recording was performed in humidified cultured box saturated with 95% O2/5% CO2. b Tracking of α-syn seeds (white dots) conducted by Image-J plug-in Trackmate. Velocity shown by line color. Bar: 500 μm. c High magnification of α-syn seed tracking. Color bar shows speeds. Bar: 50 μm (see Supplemental video 1 for their dynamics). d Velocity of QD-α-syn seeds in each area of (c). e Fluorescence view of tissue sections (10 μm thick) at 2 h after injection of QD-α-syn seed. f QD only was injected. Indicated squares in the contralateral hemisphere (middle panels) were magnified (right panels) (e, f). Bar: 500 μm (low mag.: left and middle panels), 10 μm (high mag.: right panel).
