Fig. 3: Pharmacological analysis of QD-α-syn seed dynamics.

a Experimental procedure for pharmacological analysis on acute QD-α-syn seed dynamics. Ips, ipsilateral. Con contralateral. Yellow arrow: QD-α-syn seeds injected to striatum. ROI: Region of interest. Bar: 500 μm. b–d Effect of drugs: b dynasore (Dyn); c colchicine (Col); d botulinum toxin (BoNT). Top: QD-α-syn seed number normalized by the number at pretreatment (Pre) with solvent only. For dynasore, DMSO was used for dilution of dynasore (b), and EtOH was used for dilution of colchicine (c). The same concentration of DMSO or EtOH were added in the solution for pretreatment. Bottom: mean velocity of QD-α-syn seeds normalized by that of pretreatment. Each line indicates the result from the same slice, respectively (n = 4–6 mice). e–h Drug effects on the axonal transport of QD-α-syn-seeds. e, f Effect of Chil D, dynein inhibitor (retrograde transport inhibitor) on QD-α-syn seeds transport (e α-syn number, f mean velocity). g, h Effect of STLC, kinesin inhibitor (anterograde transport inhibitor) on QD-α-syn seeds transport (g α-syn number, h mean velocity). n = 4–6 mice (Chil D), n = 5 mice (STLC). p < 0.05: statistically significant by one-way ANOVA followed by Tukey’s post test.