Fig. 4: QD-α-syn seed dynamics depend on neuronal activity.

Effect of drugs bath-applied on QD-α-syn seed dynamics in the slice. a GABA, b bicuculline (GABAa receptor antagonist), c ampakine (AMPA receptor agonist), d DNQX(AMPA receptor antagonist), e AP5 (NMDA receptor antagonist), and f TTX, tetrodotoxin. Top panels: number of QD-α-syn seeds normalized by pretreatment. Bottom panels: mean velocity of QD-α-syn seeds normalized by that of pretreatment. Experimental procedure and ROI are the same as in Fig. 3a. n = 4–6 mice. p < 0.05: statistically significant by one-way ANOVA followed by Tukey’s post test.