Fig. 2: OI promoted the expression of autophagy markers and inhibited the expression of apoptosis-related proteins in C28/I2 cells. | Communications Biology

Fig. 2: OI promoted the expression of autophagy markers and inhibited the expression of apoptosis-related proteins in C28/I2 cells.

From: Four-octyl itaconate improves osteoarthritis by enhancing autophagy in chondrocytes via PI3K/AKT/mTOR signalling pathway inhibition

Fig. 2

a–c qRT-PCR was used to analyse the mRNA expression of LC3, Beclin-1, and p62 in chondrocytes treated with IL-1β and OI (n = 3). d–g WB was performed to analyse the expression of Beclin-1, LC3 and p62, which were normalised to GAPDH (n = 3). h, i Western blotting analysis of BAX, pro-caspase3 and cleaved caspase3 protein expression normalised to GAPDH (n = 3). Internal control was performed using GAPDH. The error bar is SD. The data are the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.

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