Fig. 2: The transgene integration site and its flanking sequence from PE-WGS and PCR analyses. | Communications Biology

Fig. 2: The transgene integration site and its flanking sequence from PE-WGS and PCR analyses.

From: A paired-end whole-genome sequencing approach enables comprehensive characterization of transgene integration in rice

Fig. 2

a IGV view of 69 candidate read pairs crossing the insertion site of chromosome 3. The red line indicates the transgene insertion site in the rice genome. b Location of the transgene integration site according to the assembled contigs from candidate read pairs. c Conventional PCR amplification results for the flanking sequence. Lane M, DNA marker with six DNA fragments of 100, 250, 500, 750, 1000, and 2000 bp, respectively; Lane GM, G281; Lane WT, Xiushui 110; Lane NTC, no template control.

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