Table 2 Extracted read pairs crossing the transgene insertion site derived from original and modified TranSeq pipeline in G281 rice and WT.

From: A paired-end whole-genome sequencing approach enables comprehensive characterization of transgene integration in rice

 

G281

Reason

WT (Xiushui 110)

Reason

 

Not filtered

Filtered

 

Not Filtered

Filtered

 

Chr 01

17

0

Not really positivesa

17

0

Not really positivesa

Chr 02

75

2

Not really positivesb

94

4

Not really positivesb

Chr 03

70

62

candidate

9

0

Not really positivesb

Chr 04

1

1

noise sequences/alignment jitter

0

0

 

Chr 06

1

1

noise sequences/alignment jitter

0

0

 

Chr 07

0

0

 

1

1

noise sequences/alignment jitter

Chr 08

1

1

noise sequences/alignment jitter

0

0

 

Chr 10

155

0

Not really positivesa

141

0

Not really positivesa

Chr 11

1

1

noise sequences/alignment jitter

0

0

 

Chr 12

1

1

noise sequences/alignment jitter

0

0

 
  1. aHomologous sequences between Gt1 promoter and rice genome.
  2. bHomologous sequences between CYP81A6 RNAi and rice genome.