Table 3 Estimation of the copy number of transgenes in rice line G281 using resequencing data and ddPCR.

From: A paired-end whole-genome sequencing approach enables comprehensive characterization of transgene integration in rice

Gene

PE-WGS

ddPCR assay

G281

 

WT

Copy number

Quantified copies

RSD (%)

Copy number

ADTgm

Dgm

Rgm

 

ADTwt

Dwt

Rwt

1

2

3

Mean

HLF

48.93

28.91

0.989

 

0

29.28

0.989

1.95

37160

37760

37150

37356.7

0.94

1.96

EPSPS

53.76

28.91

0.989

 

0

29.28

0.989

2.14

35680

35800

35320

35600

0.70

1.87

Gt1

77.75

28.91

0.989

 

27.05

29.28

0.989

2.02

/

/

/

/

/

/

CYP81A6 RNAi

50.86

28.91

0.989

 

0.59

29.28

0.989

2.00

/

/

/

/

/

/

Ubiquitin

43.87

28.91

0.989

 

0.79

29.28

0.989

1.72

/

/

/

/

/

/

PEPC

43.97

28.91

0.989

 

0

29.28

0.989

1.75

/

/

/

/

/

/

SPS

25.11

28.91

0.989

 

25.14

29.28

0.989

/

18980

19000

19100

19026.7

0.34

/

  1. “Gt1”,rice glutelin promoter. “ CYP81A6 RNAi”, including a 3′ untranslated region (UTR), the RNAi sequence, and the CaMV35s promoter.“/” means did not detect.
Back to article page