Table 4 Extracted read pairs crossing the Gt1 inserted site derived from original and modified TranSeq pipeline in artificial cisgenesis rice and WT.

From: A paired-end whole-genome sequencing approach enables comprehensive characterization of transgene integration in rice

 

Artificial cisgenesis

Reason

WT (Xiushui 110)

Reason

Not filtered

Filtered

Not filtered

Filtered

Chr 01: 32076358-32077341

20

20

Not really positivesa

20

20

Not really positivesa

Chr 01: 32076840-32078305

103

0

Not really positivesa

103

0

Not really positivesa

Chr 01: 32078413-32078798

13

13

Not really positivesa

13

13

Not really positivesa

Chr 04: 675093

1

1

noise sequences/alignment jitter

1

1

noise sequences/alignment jitter

Chr 05: 11030976-11031684

116

116

candidate

0

0

 

Chr 10: 13497001-13497784

65

65

Not really positivesa

65

65

Not really positivesa

Chr 10: 13497378-13499421

1008

0

Not really positivesa

508

0

Not really positivesa

Chr 10: 13499431-13499725

56

56

Not really positivesa

56

56

Not really positivesa

  1. a Homologous sequence between Gt1 and rice genome.