Fig. 2: Peptidomimetic inhibitors block activity of purified proteases.
From: Peptidomimetic inhibitors of TMPRSS2 block SARS-CoV-2 infection in cell culture
Isolated TMPRSS2 (a), matriptase (b), thrombin (c), and factor Xa (d) were mixed with peptidomimetic inhibitors, camostat mesylate (CM), and FOY-251. After 30 min, the fluorogenic reference substrate Boc-Gln-Ala-Arg-AMC was added to TMPRSS2 or matriptase and the chromogenic substrates d-Phe-Homopro-Arg-pNA or Bz-Ile-Glu-Gly-Arg-pNA were added to thrombin or factor Xa, respectively. The velocity of substrate degradation was assessed by recording the fluorescence intensity at 460 nm or the absorbance at 405 nm within 2 h. Shown are the means ± SD of n = 1 experiment performed in triplicates.
