Fig. 6: Peptidomimetic inhibitors reduce SARS-CoV-2 infection.
From: Peptidomimetic inhibitors of TMPRSS2 block SARS-CoV-2 infection in cell culture
Peptidomimetic inhibitors and the small molecule camostat mesylate (CM) were added to Caco-2 cells. After 1 h, cells were infected with SARS-CoV-2 Wuhan-Hu-1 (a), SARS-CoV-2 bearing the spike D614G mutation (b), or the variants of concern Alpha (c) and Beta (d). Infection rates were determined 2 days post infection by in cell ELISA for the viral N protein. (e) Cytotoxicity of peptidomimetic inhibitors. Inhibitors and the small molecule camostat mesylate (CM) were added to Caco-2 cells. Cell viability was assessed 2 days post addition by measuring ATP content in cell lysates. Due to low stock concentration compounds 1, 6, and 8 were tested at a maximum concentration of 20,000 nM. Shown are the means ± SEM of n = 3 independent experiments (a–d) or mean ± SD of n = 1 experiment (e), each performed in triplicates. Calculated IC50 values for each compound are listed in Supplementary Table 8.
