Fig. 2: Parental PNs compete for chromatin dynamics promoting factors.
From: Parental competition for the regulators of chromatin dynamics in mouse zygotes
a Illustration of the preparation of 1, 2, and 4PN partheno-zygotes and fluorescence images of the fPNs. Sr indicates strontium, which induces oocyte activation. b Average MF scores of the partheno-zygotes prepared as shown in a. n = 66, 43, 19, respectively. c Illustration of the preparation of sp-mPN-enucleated zygotes. d Average MF scores of the zygotes prepared as shown in c. Blue, mPN; pink, fPN-8h; orange, fPN-10h; and red, fPN-11h). Asterisks indicate significant differences. Illustration of the preparation of delay ICSI-zygotes. n = 24, 24, 26, 30, 29, 26, respectively. e Illustration of the preparation of delay ICSI-zygotes. f Control and delay ICSI are shown. The second polar body is indicated with a red arrow. Fluorescence images of the mPN and fPN. The inset shows lower magnification images of the second polar body near the fPN. g Average MF scores of the delay ICSI-zygotes prepared as shown in e, f. n = 30, 29, 23, respectively. The error bar indicates SE.
