Fig. 8: Pseudotyped and authentic virus neutralization assays. | Communications Biology

Fig. 8: Pseudotyped and authentic virus neutralization assays.

From: A panel of nanobodies recognizing conserved hidden clefts of all SARS-CoV-2 spike variants including Omicron

Fig. 8: Pseudotyped and authentic virus neutralization assays.The alternative text for this image may have been generated using AI.

a Means of the number of infectious units of virus for the SARS-CoV-2 spike Omicron variants (n = 3). Human osteosarcoma (HOS) cells expressing ACE2 and TMPRSS2 infected with HIV-based pseudotyped virus were assessed. Relative infection units at the indicated concentrations of the P86 (black) and sotrovimab (grey) are plotted; means ± standard deviation (SD) are shown. Measured IC50s for the SARS-CoV-2 Omicron variants BA.1 and BA.2 of P86 and sotrovimab are inset. b, c Concentration dependency of the P86 nanobody (b) and sotrovimab antibody (c) for plaque reduction of the authentic SARS-CoV-2 Omicron BA.1 variant virus. The ratios of plaque formation are expressed as a percentage of the plaque numbers from untreated cells and fitted with a sigmoidal function using Igor Pro (ver. 8.04, Wavemetrics). Data are representative of two independent experiments. d The C246 nanobody versus plaque formation of the authentic Wuhan and Omicron BA.1 variant viruses. The ratios of plaque formation are shown as a percentage of the plaque numbers from untreated cells (n = 1).

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